This course, for graduate students, encompasses topics in fundamental and applied photochemistry and photophysics from the fields of organic chemistry and chemical biology. Key topics and concepts will include basic photophysics, interactions of light with matter, UV-Vis absorption and emission spectroscopy, energy transfer, kinetics/dynamics, Jablonski diagrams, electron transfer, organic photochemistry, and applications in organic chemistry and chemical biology. These topics and concepts will be covered in the context of frontier applications including synthetic chemistry organic photochemistry, molecular imaging, and optogenetic tools among others.